半胱酰化及三硫键分析

Characterization of cysteinylation and trisulfide bonds in a recombinant monoclonal antibody

  1. The peak with the molecular weight of 148,850Da is 240 Da higher than the main peak, which corresponds to the antibody with cysteinylation of two cysteine residues.
  2. The molecular weight of the main peak shoulder cannot be accurately determined, but it is approximately 28-34 Da higher than the main peak. It was hypothesized that this shoulder was due to the presence of the trisulfide bond that increases the molecular weight by 32 Da. This shoulder disappeared after a brief reduction with free cysteine, further supporting that it is formed due to trisulfide bond.
3.IdeS也酶切位点